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chir 99021  (Fisher Scientific)

 
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    Structured Review

    Fisher Scientific chir 99021
    Chir 99021, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/chir+99021/99021+chir/pmc13054417-45-0-3
    Average 86 stars, based on 1 article reviews
    chir 99021 - by Bioz Stars, 2026-10
    86/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Imaging Cellular Metabolic Rewiring with SuMMIT-SRS
    Article Snippet: Plates for transdifferentiation were coated with 10 μg/mL poly-D-lysine (PDL; Sigma #P6407-5MG) and 0.0001% (w/v) poly-L-ornithine (PLO; Sigma #P4957-50ML) for 24 h and 10 μg/mL laminin (Sigma #L2020-1MG) for an additional 24 h. The coated plates were seeded with 300% confluent UNA fibroblasts. .. Transdifferentiating neurons were cultured for three weeks in NK media (NK) (0.5X DMEM/F-12, GlutaMAX supplement, Life #10565042; 0.5X Neurobasal, Life #21103049; 1X B-27 supplement, Life #17504044; 1X N-2 supplement, Life #17502048; 20 μg/mL laminin; 400 μg/mL dibutyryl-cAMP (db-cAMP), SelleckChem #S7858; 2 μg/L doxycycline, Sigma #D9881-10G; 5 μM dorsomorphine homolog 1 (DMH1), Fisher #412610; 0.5 μM LDN-193189, Fisher #50176042; 0.5 μM A83-01, Stem Cell Technologies #72022; 5 μM forskolin, Stem Cell Technologies #72114; 3 μM CHIR-99021, Fisher #442310; 10 μM SB-431542, Fisher #161410; 10 U/mL penicillin-streptomycin, Thermo #15140122) with media changes every 48-72 h. Mature neurons were passaged to laminin-coated coverslips (Fisher Scientific #NC0937501) for Raman spectroscopy or PDL/PLO/laminin-treated Ibidi chambers (Ibidi #81811) for immunofluorescence and maintained in BrainPhys+GF media (BP-GF) (1X BrainPhys, Stem Cell Technologies #05790; 1X B-27 supplement; 1X N-2 supplement; 400 μg/mL db-cAMP; 1 μg/mL laminin; 20 ng/mL BDNF, PeproTech #450-02-50UG; and 20 ng/mL GDNF, PeproTech #450-10-50UG) for 1 week until imaging. .. Transdifferentiation progress was monitored using a Zeiss Axio-Vert.A1, which was also used to acquire brightfield images with a 20x objective lens.

    Raman Spectroscopy:

    Article Title: Imaging Cellular Metabolic Rewiring with SuMMIT-SRS
    Article Snippet: Plates for transdifferentiation were coated with 10 μg/mL poly-D-lysine (PDL; Sigma #P6407-5MG) and 0.0001% (w/v) poly-L-ornithine (PLO; Sigma #P4957-50ML) for 24 h and 10 μg/mL laminin (Sigma #L2020-1MG) for an additional 24 h. The coated plates were seeded with 300% confluent UNA fibroblasts. .. Transdifferentiating neurons were cultured for three weeks in NK media (NK) (0.5X DMEM/F-12, GlutaMAX supplement, Life #10565042; 0.5X Neurobasal, Life #21103049; 1X B-27 supplement, Life #17504044; 1X N-2 supplement, Life #17502048; 20 μg/mL laminin; 400 μg/mL dibutyryl-cAMP (db-cAMP), SelleckChem #S7858; 2 μg/L doxycycline, Sigma #D9881-10G; 5 μM dorsomorphine homolog 1 (DMH1), Fisher #412610; 0.5 μM LDN-193189, Fisher #50176042; 0.5 μM A83-01, Stem Cell Technologies #72022; 5 μM forskolin, Stem Cell Technologies #72114; 3 μM CHIR-99021, Fisher #442310; 10 μM SB-431542, Fisher #161410; 10 U/mL penicillin-streptomycin, Thermo #15140122) with media changes every 48-72 h. Mature neurons were passaged to laminin-coated coverslips (Fisher Scientific #NC0937501) for Raman spectroscopy or PDL/PLO/laminin-treated Ibidi chambers (Ibidi #81811) for immunofluorescence and maintained in BrainPhys+GF media (BP-GF) (1X BrainPhys, Stem Cell Technologies #05790; 1X B-27 supplement; 1X N-2 supplement; 400 μg/mL db-cAMP; 1 μg/mL laminin; 20 ng/mL BDNF, PeproTech #450-02-50UG; and 20 ng/mL GDNF, PeproTech #450-10-50UG) for 1 week until imaging. .. Transdifferentiation progress was monitored using a Zeiss Axio-Vert.A1, which was also used to acquire brightfield images with a 20x objective lens.

    Immunofluorescence:

    Article Title: Imaging Cellular Metabolic Rewiring with SuMMIT-SRS
    Article Snippet: Plates for transdifferentiation were coated with 10 μg/mL poly-D-lysine (PDL; Sigma #P6407-5MG) and 0.0001% (w/v) poly-L-ornithine (PLO; Sigma #P4957-50ML) for 24 h and 10 μg/mL laminin (Sigma #L2020-1MG) for an additional 24 h. The coated plates were seeded with 300% confluent UNA fibroblasts. .. Transdifferentiating neurons were cultured for three weeks in NK media (NK) (0.5X DMEM/F-12, GlutaMAX supplement, Life #10565042; 0.5X Neurobasal, Life #21103049; 1X B-27 supplement, Life #17504044; 1X N-2 supplement, Life #17502048; 20 μg/mL laminin; 400 μg/mL dibutyryl-cAMP (db-cAMP), SelleckChem #S7858; 2 μg/L doxycycline, Sigma #D9881-10G; 5 μM dorsomorphine homolog 1 (DMH1), Fisher #412610; 0.5 μM LDN-193189, Fisher #50176042; 0.5 μM A83-01, Stem Cell Technologies #72022; 5 μM forskolin, Stem Cell Technologies #72114; 3 μM CHIR-99021, Fisher #442310; 10 μM SB-431542, Fisher #161410; 10 U/mL penicillin-streptomycin, Thermo #15140122) with media changes every 48-72 h. Mature neurons were passaged to laminin-coated coverslips (Fisher Scientific #NC0937501) for Raman spectroscopy or PDL/PLO/laminin-treated Ibidi chambers (Ibidi #81811) for immunofluorescence and maintained in BrainPhys+GF media (BP-GF) (1X BrainPhys, Stem Cell Technologies #05790; 1X B-27 supplement; 1X N-2 supplement; 400 μg/mL db-cAMP; 1 μg/mL laminin; 20 ng/mL BDNF, PeproTech #450-02-50UG; and 20 ng/mL GDNF, PeproTech #450-10-50UG) for 1 week until imaging. .. Transdifferentiation progress was monitored using a Zeiss Axio-Vert.A1, which was also used to acquire brightfield images with a 20x objective lens.

    Imaging:

    Article Title: Imaging Cellular Metabolic Rewiring with SuMMIT-SRS
    Article Snippet: Plates for transdifferentiation were coated with 10 μg/mL poly-D-lysine (PDL; Sigma #P6407-5MG) and 0.0001% (w/v) poly-L-ornithine (PLO; Sigma #P4957-50ML) for 24 h and 10 μg/mL laminin (Sigma #L2020-1MG) for an additional 24 h. The coated plates were seeded with 300% confluent UNA fibroblasts. .. Transdifferentiating neurons were cultured for three weeks in NK media (NK) (0.5X DMEM/F-12, GlutaMAX supplement, Life #10565042; 0.5X Neurobasal, Life #21103049; 1X B-27 supplement, Life #17504044; 1X N-2 supplement, Life #17502048; 20 μg/mL laminin; 400 μg/mL dibutyryl-cAMP (db-cAMP), SelleckChem #S7858; 2 μg/L doxycycline, Sigma #D9881-10G; 5 μM dorsomorphine homolog 1 (DMH1), Fisher #412610; 0.5 μM LDN-193189, Fisher #50176042; 0.5 μM A83-01, Stem Cell Technologies #72022; 5 μM forskolin, Stem Cell Technologies #72114; 3 μM CHIR-99021, Fisher #442310; 10 μM SB-431542, Fisher #161410; 10 U/mL penicillin-streptomycin, Thermo #15140122) with media changes every 48-72 h. Mature neurons were passaged to laminin-coated coverslips (Fisher Scientific #NC0937501) for Raman spectroscopy or PDL/PLO/laminin-treated Ibidi chambers (Ibidi #81811) for immunofluorescence and maintained in BrainPhys+GF media (BP-GF) (1X BrainPhys, Stem Cell Technologies #05790; 1X B-27 supplement; 1X N-2 supplement; 400 μg/mL db-cAMP; 1 μg/mL laminin; 20 ng/mL BDNF, PeproTech #450-02-50UG; and 20 ng/mL GDNF, PeproTech #450-10-50UG) for 1 week until imaging. .. Transdifferentiation progress was monitored using a Zeiss Axio-Vert.A1, which was also used to acquire brightfield images with a 20x objective lens.



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    Image Search Results


    Modulation of Wnt/β-catenin signaling is associated with changes in MGMT expression following 20 (S) -PPD treatment. (A) β-catenin and MGMT protein levels after β-catenin knockdown in LN18 and T98G cells, detected by western blotting ( n = 3). (B) Cell viability after TMZ treatment in β-catenin-silenced cells or scrambled shRNA controls, measured by CCK-8 assay ( n = 3). (C) CCK-8 analysis of TMZ-treated β-catenin-knockdown cells with or without MGMT overexpression ( n = 3). (D) Wnt3a, GSK3β, p-GSK3β (Ser9), β-catenin, and MGMT protein abundance after 24-h exposure to increasing concentrations of 20 (S) -PPD, assessed by western blotting ( n = 3). (E) Effects of CHIR-99021 on GSK3β, p-GSK3β (Ser9), β-catenin, and MGMT levels in 20 (S) -PPD-treated cells, examined by western blotting ( n = 3). (F) Changes in β-catenin and MGMT protein levels after β-catenin overexpression under 20 (S) -PPD treatment, determined by western blotting ( n = 3). (G) CCK-8 analysis of cell viability after combined 20 (S) -PPD and TMZ treatment in β-catenin-overexpressing LN18 and T98G cells ( n = 3). ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: Frontiers in Medicine

    Article Title: 20(S)-protopanaxadiol enhances temozolomide responsiveness in MGMT-expressing glioblastoma: Wnt/β-catenin attenuation and MGMT downregulation

    doi: 10.3389/fmed.2026.1911347

    Figure Lengend Snippet: Modulation of Wnt/β-catenin signaling is associated with changes in MGMT expression following 20 (S) -PPD treatment. (A) β-catenin and MGMT protein levels after β-catenin knockdown in LN18 and T98G cells, detected by western blotting ( n = 3). (B) Cell viability after TMZ treatment in β-catenin-silenced cells or scrambled shRNA controls, measured by CCK-8 assay ( n = 3). (C) CCK-8 analysis of TMZ-treated β-catenin-knockdown cells with or without MGMT overexpression ( n = 3). (D) Wnt3a, GSK3β, p-GSK3β (Ser9), β-catenin, and MGMT protein abundance after 24-h exposure to increasing concentrations of 20 (S) -PPD, assessed by western blotting ( n = 3). (E) Effects of CHIR-99021 on GSK3β, p-GSK3β (Ser9), β-catenin, and MGMT levels in 20 (S) -PPD-treated cells, examined by western blotting ( n = 3). (F) Changes in β-catenin and MGMT protein levels after β-catenin overexpression under 20 (S) -PPD treatment, determined by western blotting ( n = 3). (G) CCK-8 analysis of cell viability after combined 20 (S) -PPD and TMZ treatment in β-catenin-overexpressing LN18 and T98G cells ( n = 3). ns, not significant; * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: TMZ (CAS No. 85622-93-1, Cat. No. HY-17364, purity 99.96%) and CHIR-99021 (CAS No. 252917-06-9, Cat. No. HY-10182, purity 99.46%) were obtained from MedChemExpress (Shanghai, China).

    Techniques: Expressing, Knockdown, Western Blot, shRNA, CCK-8 Assay, Over Expression, Quantitative Proteomics